vl region dna (Thermo Fisher)

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Thermo Fisher vl region dna
Vl Region Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vl+region+dna/DNA/us10047148-1555-10-18
Average 99 stars, based on 1 article reviews

vl region dna - by Bioz Stars, 2026-09

99/100 stars

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Polymerase Chain Reaction:

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μ15× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 ng of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Sequencing:

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 ng of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Cloning:

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 ng of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Expressing:

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 ng of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Plasmid Preparation:

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5×Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs are performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA are ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 μg of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, is mixed with 40 μl FUGENE® 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

Article Title: Neutralizing GP41 antibodies and their use
Article Snippet: All PCRs were performed in 96-well PCR plates in a total volume of 50 μl containing 20 nM each primer or primer mix, 10 nM each dNTP (Invitrogen), 10 μl 5× Q-solution (Qiagen) and 1.2 U HotStar Taq DNA polymerase (Qiagen). .. From the positive PCR reactions, pools of the VH or VL-region DNA were ligated to a pCR2.1-Topo-TA vector (Invitrogen) for sequencing before cloning into the corresponding Igγ1, Igκ and Igλ expression vector. .. 10 ng of heavy and light chain plasmids, cloned from the same well and combined in all possible heavy and light chain pairs, were mixed with 40 μl FuGENE 6 (Roche) in 1500 μl DMEM (Gibco) and co-transfected into 293T cells.

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